A novel sequential electrostatic-HILIC SPE within a quantitative method for bivalirudin in human plasma by LC–SRM. (November 2016)

• Record Type: Journal Article
• Authors: MacNeill, Robert; Acharya, Vishva; Patel, Neha; Parisi, Gregory; Katto, Kim; Lovich, Robert
• Is Part Of: Bioanalysis. Volume 8:Number 21(2016)
• Page Start: 2195
• Page End: 2203
• Publication Date: 2016-11
• Subjects: bivalirudin -- electrostatic -- hydrophilic; Drugs -- Analysis -- Periodicals; 615.19005
• DOI: 10.4155/bio-2016-0175
• Languages: English
• ISSNs: 1757-6180

Abstract

Aim: This work set out to realize an idea for a novel means of extracting the peptide therapeutic bivalirudin from human plasma in what would be a uniquely selective means of SPE, a mixed-mode protocol involving electrostatic interactions followed by HILIC.Results: Inter and intra-assay relative error ranged from 3.52 to 8.23%, and 2.37 to 6.90%, respectively. Inter and intra-assay precision ranged from 2.64 to 7.12%, and 0.855 to 2.90%, respectively. Recoveries of 80% were attained, and there was no hint of discernible manifestation of matrix effects.Conclusion: The method was shown to perform excellently in the assessment tantamount to method validation. The essence of the extraction method presents a new option for highly selective extraction of peptides from biological matrices.