Cation-dependent mannose-6-phosphate receptor functions as a pattern recognition receptor in anti-bacterial immunity of Marsupenaeus japonicus. (December 2018)

• Record Type: Journal Article
• Authors: Zhang, Ke-Yi; Yuan, Wen-Jie; Xu, Ji-Dong; Wang, Jin-Xing
• Is Part Of: Developmental and comparative immunology. Volume 89(2018)
• Page Start: 122
• Page End: 130
• Publication Date: 2018-12
• Subjects: Cation-dependent mannose-6-phosphate receptor -- Bacterium -- Antimicrobial peptide -- Polysaccharides; Immunology -- Periodicals; Developmental immunology -- Periodicals; 616.079
• DOI: 10.1016/j.dci.2018.08.011
• Languages: English
• ISSNs: 0145-305X

Abstract

Abstract: The cation-dependent mannose-6-phosphate receptor (CD-MPR) is a member of the P-type lectin family. As a type I transmembrane glycoprotein, it functions in the delivery of newly synthesized acid hydrolases from the trans-Golgi network to endosomes for their subsequent transfer to the lysosome by binding the mannose-6-phosphate receptor-recognition moieties in the hydrolases. However, the functions of CD-MPR in immune responses are seldom reported. In the present study, we identified a CD-MPR-like molecule in Marsupenaeus japonicus and designed it as Mj CD-MPR. It was significantly upregulated after challenge with Vibrio anguillarum at the mRNA and protein levels. Knockdown of MjCD-MPR resulted in a significant increase in the amount of V. anguillarum in the hemolymph of shrimp, which suggested that Mj CD-MPR plays a role in shrimp antibacterial defense. The recombinant extracytoplasmic region of Mj CD-MPR could bind gram-positive and gram-negative bacteria by interaction with peptidoglycan, lipopolysaccharide, and lipoteichoic acid. Mj CD-MPR showed no direct bacteriostatic or bacteriocidal activity. Knockdown of MjCD-MPR decreased the expression levels of several antimicrobial peptides ( Alf-C1, Alf-E1, Crustin I-2, and Crustin I-3 ), suggesting that Mj CD-MPR promotes the expression of antimicrobial peptides in shrimp. In summary, working as a pattern recognition receptor, Mj CD-MPR recognizes invading bacteria and triggers the expression of AMPs against bacterial infection in shrimp. Highlights: A CD-MPR identified in Marsupenaeus japonicus is upregulated in shrimp challenged by bacteria. Mj CD-MPR promotes in vivo bacterial clearance and plays a role against bacterial infection in shrimp. The extracytoplasmic region of Mj CD-MPR binds gram-positive and gram-negative bacteria by binding to polysaccharides. Mj CD-MPR plays a role against bacterial infection by promoting the expression of antimicrobial peptides.