Assessment of the oxidative and genotoxic effects of the glyphosate-based herbicide roundup on the freshwater shrimp, Macrobrachium nipponensis. (November 2018)

• Record Type: Journal Article
• Authors: Hong, Yuhang; Yang, Xiaozhen; Huang, Yi; Yan, Guangwen; Cheng, Yongxu
• Is Part Of: Chemosphere. Volume 210(2018)
• Page Start: 896
• Page End: 906
• Publication Date: 2018-11
• Subjects: Roundup -- Macrobrachium nipponensis -- Oxidative stress -- Genotoxicity; Pollution -- Periodicals; Pollution -- Physiological effect -- Periodicals; Environmental sciences -- Periodicals; Atmospheric chemistry -- Periodicals; 551.511
• DOI: 10.1016/j.chemosphere.2018.07.069
• Languages: English
• ISSNs: 0045-6535

Abstract

Abstract: In the present study, an acute toxic test was performed to assess the oxidative stress and genotoxic effects of the herbicide on the freshwater shrimp Macrobrachium nipponensis . The results showed that the 48-h and 96-h LC50 values of Roundup to M. nipponensis were 57.684 mg/L and 11.237 mg/L, respectively. For further investigation, the shrimps were exposed to sublethal concentrations of 0.35, 0.70, 1.40, 2.80 and 5.60 mg/L for 96 h. A significant decrease in total haemocytes count (THC) was observed at concentration of 5.60 mg/L throughout the experiment. The level of superoxide dismutase (SOD), catalase (CAT) and total antioxidant capacity (T-AOC) in all the treatments decreased in a dose- and time-dependent manner except for the concentration group of 0.35 mg/L. The malondialdehyde (MDA), hydrogen peroxide (H2 O2 ) and protein carbonyl in serum increased significantly at concentrations of 2.80 mg/L and 5.60 mg/L. A significant decrease in acetylcholinesterase (AChE) activity was observed at each concentration ( P ＜0.05). In addition, the micronucleus (MN) frequency of haemocytes significantly increased ( P ＜0.05) at concentrations of 1.40, 2.80 and 5.60 mg/L, whereas the comet ratio and %DNA in the tails exhibited a clear time- and dose-dependent response during the exposure. The analysis of the integrated biomarker response (IBR) showed the induction of oxidative stress biomarkers and the inhibition of antioxidants, and this dose-dependent relation suggests the sensitivity and availability of all the biomarkers. These results revealed that Roundup had a prominent toxic effect on M. nipponensis based on the antioxidative response inhibition and genotoxicity. Highlights: An acute toxic test was conducted to evaluate the effect of Roundup on oxidative stress and genotoxicity from Macrobrachium nipponensis . Roundup could induce significant oxidative stress and genotoxic effect by sublethal concentration exposure. All biomarkers except CAT were considered sensitive for toxicity assessment of Roundup exposure.