Acute Aversive Stimuli Rapidly Increase the Activity of Ventral Tegmental Area Dopamine Neurons in Awake Mice. (21st August 2018)

• Record Type: Journal Article
• Authors: Moriya, Shunpei; Yamashita, Akira; Kawashima, Shigetaka; Nishi, Ryusei; Yamanaka, Akihiro; Kuwaki, Tomoyuki
• Is Part Of: Neuroscience. Volume 386(2018)
• Page Start: 16
• Page End: 23
• Publication Date: 2018-08-21
• Subjects: AAV adeno associated virus -- CaM calmodulin -- DA dopamine -- DAT dopamine transporter -- GFP green fluorescent protein -- NAc nucleus accumbens -- PBS phosphate-buffered saline -- PFC prefrontal cortex -- TMT 2, 4, 5-trimethyl thiazoline -- VTA ventral tegmental area; G-CaMP6 -- fiber photometry -- tail pinch -- ultrasonic sound -- predator odor -- intruder mouse; Neurochemistry -- Periodicals; Neurophysiology -- Periodicals; Neurology -- Periodicals; Neurochimie -- Périodiques; Neurophysiologie -- Périodiques; Neurochemistry; Neurophysiology; Electronic journals; Periodicals; Electronic journals; 612.8
• DOI: 10.1016/j.neuroscience.2018.06.027
• Languages: English
• ISSNs: 0306-4522

Abstract

Graphical abstract: Highlights: The ventral tegmental area (VTA) is one of the nuclei of the dopaminergic systems and is involved in reward processing. We utilized a fiber photometry system to specifically investigate the activity of dopamine (DA) neurons in the VTA. Noxious and non-noxious aversive stimuli rapidly and transiently activated VTA DA neurons while control stimuli did not. VTA DA neurons are involved in an aversive sensory processing in addition to well-known reward processing. Abstract: The ventral tegmental area (VTA) is one of the origins of the brain dopaminergic system and is involved in regulating various physiological functions such as pain processing and motivation. In this study, we utilized a fiber photometry system to specifically investigate the activity of dopamine neurons in the VTA using dopamine transporter promoter-driven Cre recombinase-expressing mice and site-specific infection of adeno-associated virus carrying the FLEX G-CaMP6 gene. As expected, expression of G-CaMP6 was restricted to VTA dopamine neurons. We recorded G-CaMP6 green fluorescent signal, which reflected dopaminergic neuronal activity, in awake mice exposed to tail pinch, ultrasonic sound, predator odor, and a male intruder mouse. These stimuli resulted in a rapid and short-lasting increase in the activity of VTA dopamine neurons while the control stimuli of a gentle tail touch and appearance of empty box did not induce any changes. In addition, fluorescence intensity was not changed by any of these stimuli in the control animals expressing hrGFP instead of G-CaMP6 in VTA dopamine neurons. Our data clearly show that acute aversive stimuli rapidly increase the activity of VTA dopamine neurons and thus suggest a salience-processing role.