Biocompatible hybrids based on nanographene oxide covalently linked to glycolporphyrins: Synthesis, characterization and biological evaluation. (August 2018)

• Record Type: Journal Article
• Authors: Santos, Carla I.M.; Gonçalves, Gil; Cicuéndez, Mónica; Mariz, Inês; Silva, Virgília S.; Oliveira, Helena; Campos, Fábio; Vieira, Sandra I.; Marques, Paula A.A.P.; Maçôas, Ermelinda M.S.; Neves, M.Graça P.M.S.; Martinho, José M.G.
• Is Part Of: Carbon. Volume 135(2018)
• Page Start: 202
• Page End: 214
• Publication Date: 2018-08
• Subjects: Carbon -- Periodicals; Carbone -- Périodiques; Koolstof; Toepassingen; Electronic journals; 546.681
• DOI: 10.1016/j.carbon.2018.04.040
• Languages: English
• ISSNs: 0008-6223

Abstract

Abstract: The major limitation in the development of hybrids based on graphene oxide (GO) and porphyrins is their dispersibility and stability in aqueous systems due to the hydrophobic character induced by porphyrins. Most of the previous approaches reported the direct functionalization of GO with polyethylene glycol (PEG) chains followed by the self-assembly of porphyrins by π-π interactions. Here, new hybrids were prepared using porphyrins previously functionalized with different number/types of glycol branches to be covalently attached through esterification to the carboxyl groups of GO sheets of nanometric dimensions. The number of the glycol chains and its relative position in the porphyrin core showed to be fundamental to improve the hybrids dispersion and stability in aqueous solutions. The best performing hybrids were characterized by transmission electron microscopy, X-ray photoelectron spectroscopy, Fourier transform infrared, UV-Vis absorption and fluorescence spectroscopy. The in vitro biocompatibility assessment of these hybrids was conducted using human Saos-2 cells. Their effects on cell proliferation and viability, the generation of reactive oxygen species as well as the cell morphology after cell uptake were analysed. The results demonstrate the biocompatibility of these hybrid nanomaterials with human Saos-2 cells, which is very promising for future application in biomedicine namely in cancer therapy. Graphical abstract: Image 1