Low-concentration exposure to BPA, BPF and BPAF induces oxidative DNA bases lesions in human peripheral blood mononuclear cells. (June 2018)

• Record Type: Journal Article
• Authors: Mokra, Katarzyna; Woźniak, Katarzyna; Bukowska, Bożena; Sicińska, Paulina; Michałowicz, Jaromir
• Is Part Of: Chemosphere. Volume 201(2018)
• Page Start: 119
• Page End: 126
• Publication Date: 2018-06
• Subjects: Bisphenol A -- Bisphenol F -- Bisphenol AF -- PBMCs -- DNA bases lesions; Pollution -- Periodicals; Pollution -- Physiological effect -- Periodicals; Environmental sciences -- Periodicals; Atmospheric chemistry -- Periodicals; 551.511
• DOI: 10.1016/j.chemosphere.2018.02.166
• Languages: English
• ISSNs: 0045-6535

Abstract

Abstract: Because bisphenol A (BPA) and some of its analogs have been supposed to influence development of cancer, we have assessed the effect of BPA, bisphenol S (BPS), bisphenol F (BPF) and bisphenol AF (BPAF) on DNA bases oxidation, which is a key process in cancer initiation. The analysis was conducted on human peripheral blood mononuclear cells (PBMCs), which are very useful model to assess genotoxic potential of various toxicants in different cell types. In order to determine oxidative damage to DNA pyrimidines and purines, alkaline version of the comet assay with DNA glycosylases, i.e. endonuclease III (Nth) and human 8-oxoguanine DNA glycosylase (hOGG1) was used. PBMCs were exposed to BPA or its analogs in the concentrations of 0.01, 0.1 and 1 μg/mL for 4 h and 0.001, 0.01 and 0.1 μg/mL for 48 h. We have observed that BPA, BPS, BPF and particularly BPAF caused oxidative damage to DNA pyrimidines and more strongly to purines in human PBMCs. The results have also shown that BPS, which is the most commonly used as a substitute for BPA in the manufacture induced definitely the smallest oxidative DNA bases lesions in PBMCs. Moreover, we have noticed that BPA, BPF and BPAF caused DNA damage at very low concentration of 1 ng/mL. Graphical abstract: Image Highlights: Effect of BPA and its analogs on DNA bases damage in PBMCs was studied. Bisphenols induced stronger damage to purines than pyrimidines. BPAF induced the strongest oxidative damage to DNA bases. BPS, which is main substituent of bisphenol A caused much lower DNA damage than BPA. BPA, BPF and BPAF induced damage to DNA bases even at 1 ng/mL.