Antioxidative and hepatoprotective effects of enzymatic and acidic-hydrolysis of Pleurotus geesteranus mycelium polysaccharides on alcoholic liver diseases. (1st December 2018)

• Record Type: Journal Article
• Authors: Song, Xinling; Liu, Zhonghai; Zhang, Jianjun; Zhang, Chen; Dong, Yuhan; Ren, Zhenzhen; Gao, Zheng; Liu, Min; Zhao, Huajie; Jia, Le
• Is Part Of: Carbohydrate polymers. Volume 201(2018)
• Page Start: 75
• Page End: 86
• Publication Date: 2018-12-01
• Subjects: ADH alcohol dehydrogenase -- ALD alcoholicliver diseases -- ALDH acetal dehydedehydrogenase -- ALP alkalinephosphatase -- ALT alamineaminotransferase -- L-Ara L-arabinose -- AST aspertateaminotransferase -- AcMPS acidic- hydrolysis mycelium polysaccharide -- CAT catalase -- CYP2E1 cytochrome P450 2E1 -- DMSO dimethylsulfoxide -- EnMPS enzymatic-hydrolysis mycelium polysaccharide -- L-Fuc L-fucose -- FT-IR fourier transform infrared spectroscopy -- D-Gal D-galactose -- GC gas chromatography -- D-Glc D-glucose -- GSH-Px GSH peroxide -- HDL-C high-density lipoprotein cholesterol -- LDL-C low density lipoprotein cholesterol -- LPO lipid peroxidation -- IL-1β interleukin-1β -- IL-6 interleukin-6 -- D-Man D-mannose -- MC model control -- MDA malondialdehyde -- MEOS microsomal ethanol-oxidizing system -- MPS mycelium polysaccharide -- NC normal control -- NMR nuclear magnetic resonance -- PC positive control -- Mw weight-average molecular weight -- Mn number-average molecular weight -- Mz Z- average molecular weight -- prot protein -- L-Rha L-rhamnose -- D-Rib D-ribose -- ROS reactive oxygen species -- SOD superoxide dismutase -- T-AOC total antioxidant capacity -- TC total cholesterol -- TFA trifluoroacetic acid -- TG triglyceride -- TNF-α tumor necrosis factor -- VLDL-C very low-density lipoprotein cholesterol -- D-Xyl D-xylose; Antioxidant and hepatoprotection -- Characterization -- Alcoholic liver diseases -- Enzymolysis and acidolysis -- Pleurotus geesteranus polysaccharides; Polysaccharides -- Periodicals; Polysaccharides -- Periodicals; Polysaccharides -- Périodiques; Electronic journals; 547.78
• DOI: 10.1016/j.carbpol.2018.08.058
• Languages: English
• ISSNs: 0144-8617

Abstract

Highlights: The AcMPS and EnMPS showed potential anti-oxidant effects. The polysaccharides have hepatoprotection by remitting hepatic enzymes activities. Findings indicate the bioactivities are related to physical-chemical characteristics. The polysaccharides could be functional foods and natural drugs for preventing ALD. Abstract: The present work investigated the antioxidant and hepatoprotective effects of acidic- and enzymatic-hydrolysis mycelium polysaccharide (AcMPS and EnMPS) from Pleurotus geesteranus on alcoholic liver disease (ALD) mice. The animal studies demonstrated that the polysaccharides had potential effects reflected by remitting alcoholic hepatitis, reducing lipid accumulation, preventing oxidative stress, improving inflammatory symptoms, and alleviating the liver functions by histopathologic observation. Results showed that AcMPS (yield of 84%) was composed of L-Rha, D-Rib, L-Ara, D-Glc, D-Man and D-Gal with the Mw of 3.49 × 10 4 Da, while EnMPS (yield of 90%) was contained L-Rha, L-Ara, D-Gal and D-Glc with the Mw of 3.67 × 10 4 Da. Furthermore, the GC–MS analysis indicated that both AcMPS and EnMPS were β-pyranoside polysaccharides with the (1→3)- and (1→6)-linkages. The conclusions indicated that AcMPS and EnMPS could be used as natural drugs for preventing the ALD, and providing underlying hepatoprotective mechanisms, pharmaceutically.