Lipase immobilization on amino-silane modified superparamagnetic Fe3O4 nanoparticles as biocatalyst for biodiesel production. (15th July 2018)

• Record Type: Journal Article
• Authors: Miao, Changlin; Yang, Lingmei; Wang, Zhongming; Luo, Wen; Li, Huiwen; Lv, Pengmei; Yuan, Zhenhong
• Is Part Of: Fuel. Volume 224(2018)
• Page Start: 774
• Page End: 782
• Publication Date: 2018-07-15
• Subjects: Fe3O4 magnetic nanoparticles -- Functionalization -- Immobilized lipase -- Biocatalysts -- Transesterification -- Biodiesel; Fuel -- Periodicals; Coal -- Periodicals; Coal; Fuel; Periodicals; 662.6
• DOI: 10.1016/j.fuel.2018.02.149
• Languages: English
• ISSNs: 0016-2361

Abstract

Abstract: In this work, superparamagnetic Fe3 O4 nanoparticles were synthesized by chemical co-precipitation using ionic liquid of 1-Butyl-3-methylimidazolium tetrafluoroborate ([BMIN]BF4 ) as templates. Then, the magnetic Fe3 O4 nanoparticles were treated with 3-aminopropyltriethoxysilane (APTES) and obtain the surface amino-functionalized magnetic nanoparticles (APTES-Fe3 O4 ) were used as immobilization material. Lipase was covalently bound to the APTES-Fe3 O4 magnetic nanoparticles by using glutaraldehyde as a coupling reagent. A fatty acid methyl ester (FAME) conversion of 89.4% could be achieved by lipase immobilized on APTES-Fe3 O4 magnetic nanoparticles under optimized conditions of methanol-oil molar ratio of 6:1, with a catalyst dose of 20%, 2% water content and 250 rpm agitation speed at 45 °C for 24 h. The FAME conversion remained greater than 70% even after reusing the catalyst for 5 reactions. In addition, the lipases immobilized on APTES-Fe3 O4 magnetic nanoparticles could be recovered easily by external magnetic field for further use.