Water extract of Brazilian green propolis attenuates high glucose-induced vascular morphological abnormality in zebrafish. (October 2018)

• Record Type: Journal Article
• Authors: Saito, Yuichi; Gouda, Takumi; Nakamura, Shinsuke; Ichihara, Kenji; Hara, Hideaki; Shimazawa, Masamitsu
• Is Part Of: Journal of functional foods. Volume 49(2018)
• Page Start: 250
• Page End: 257
• Publication Date: 2018-10
• Subjects: Caffeoylquinic acid -- Hyperglycemia -- Propolis -- Vascular morphology; p-CA p-coumaric acid -- CGA chlorogenic acid -- MAPK mitogen-activated protein kinase -- NAC N-acetyl cysteine -- PTU 1-phenyl-2-thiourea -- qPCR quantitative PCR -- ROP retinopathy of prematurity -- SEM standard error of the mean -- SDS sodium dodecyl sulfate -- TBA thiobarbituric acid -- TBARS thiobarbituric acid-reactive substances -- VEGF vascular endothelial growth factor -- WEP water extract of Brazilian green propolis; Functional foods -- Analysis -- Periodicals; Food -- Biotechnology -- Periodicals; Nutrition -- Periodicals; 613.2
• DOI: 10.1016/j.jff.2018.08.028
• Languages: English
• ISSNs: 1756-4646

Abstract

Graphical abstract: Highlights: Water extract of Brazilian green propolis (WEP) normalized larvae in high glucose. WEP normalized high glucose-induced abnormality of hyaloid and cerebral vessels. WEP partly suppressed high glucose-induced elevation of glucose levels in tissues. Chlorogenic acid also normalized high glucose-induced morphological abnormality. Abstract: In diabetes patients, hyperglycemia causes many vascular disorders. To clarify the effects of water extract of Brazilian green propolis (WEP) against high glucose-induced vascular disorders, we investigated using zebrafish-based vasculopathy model. Zebrafish larvae, which is transparent and small, is an ideal model organism to detect changes of the vascular structure. Zebrafish larvae were exposed to 200 mM glucose and WEP solution from 2 h post fertilization (hpf) to 3 days post fertilization (dpf). The WEP treatment normalized high glucose-induced morphological abnormality of the larvae, hyaloid vessels, and cerebral vessels. The WEP treatment partly suppressed the elevation of glucose levels and mRNA levels of p27 in tissues by exposure to high glucose content. Furthermore, we found that chlorogenic acid, which is one of the main constituents of WEP, also normalized high glucose-induced morphological abnormality. Our findings indicated that WEP improves vascular integrity in the central nervous system under high glucose conditions.