Effects of citric acid modified with fluoride, nano-hydroxyapatite and casein on eroded enamel. (September 2018)

• Record Type: Journal Article
• Authors: Dündar, Ayşe; Şengün, Abdulkadir; Başlak, Canan; Kuş, Mahmut
• Is Part Of: Archives of oral biology. Volume 93(2018)
• Page Start: 177
• Page End: 186
• Publication Date: 2018-09
• Subjects: SEM scanning electron microscopy -- AFM atomic force microscopy -- EDX energy-dispersive X-ray spectroscopy; Tooth erosion -- Casein -- Fluoride -- Nano -- Hydroxyapatite; Mouth -- Periodicals; Mouth -- Diseases -- Periodicals; Dentistry -- Periodicals; Electronic journals; 617.6005
• DOI: 10.1016/j.archoralbio.2018.06.009
• Languages: English
• ISSNs: 0003-9969

Abstract

Highlights: Modifications of citric acid with sodium fluoride reduced dental erosion. Citric acid modified with nano-hydroxyapatite reduced dental erosion. Casein modification stopped the erosive wear and repaired the enamel surface. The roughness increased after the erosion cycle in the F1, F2, and NHA1 groups. The NHA2, C1, and C2 groups, this increase was not significant. Abstract: Objectives: The aim of this in vitro study was to evaluate the effects of citric acid containing fluoride, nano-hydroxyapatite, and casein on eroded enamel. Design: The crowns of 120 extracted bovine incisors were embedded in acrylic resin. An enamel window (2 × 3 mm) was created on the surface. Before in vitro pellicle formation samples were eroded in 1% citric acid (pH = 3.2) for 1 h at 36 °C and were randomly classified to eight groups (n = 15) as follows: Positive control: 1% citric acid, Negative control: Distilled water, F1: 0.047 mmol/L sodium fluoride, F2: 0.071 mmol/L sodium fluoride, NHA1: %0.05 Nano-Hydroxyapatite, NHA2: %0.1 Nano-Hydroxyapatite, C1: %0.02 Casein, C2: %0.2 Casein. Erosion cycling was performed three times daily for 3 days. In each cycle, the samples were immersed in 10 mL of control or modified solutions (10 min) and in 10 mL of artificial saliva (60 min). The surface roughness and enamel loss were analyzed by using profilometer, scanning electron microscopy (SEM) and atomic force microscopy techniques (AFM). Results: Among the groups, the positive control group was found to be having the highest erosive wear. Erosive wear in the F2, NHA2, C1, and C2 groups was not significantly different from the negative control group (p > 0.05). The C1 and C2 groups showed that erosion terminated and minimal tissue recovery occurred on the enamel surface. Conclusion: Although all modifications reduced further demineralization, the citric acid modification with casein was found to be having a greater impact on dental erosion than the others.