Stromal morphological changes and immunophenotypic features of precancerous lesions and hepatocellular carcinoma. Issue 4 (4th January 2019)

• Record Type: Journal Article
• Authors: Chen, Dingbao; Li, Zhao; Zhu, Weihua; Cheng, Qian; Song, Qiujing; Qian, Lihua; Zhu, Ji-Ye
• Is Part Of: Journal of clinical pathology. Volume 72:Issue 4(2019)
• Page Start: 295
• Page End: 303
• Publication Date: 2019-01-04
• Subjects: hepatocellular carcinoma -- high-grade dysplastic nodule -- early hepatocellular carcinoma -- carcinogenesis -- immunohistochemistry; Pathology -- Periodicals; Pathology, Molecular -- Periodicals; 616.0705
• DOI: 10.1136/jclinpath-2018-205611
• Languages: English
• ISSNs: 0021-9746

Abstract

Abstract : Aims: To evaluate stromal histopathological features and immunostaining expression for differential diagnosis of low- and high-grade dysplastic nodules (HGDN) to early and progressed hepatocellular carcinomas (eHCC, pHCC). Materials: We evaluated sinusoid capillarisation (SC), solitary artery (SA), ductular reaction (DR), stromal invasion and expression of six biomarkers (GPC3, HSP70, GS, CD34, CK19, EpCAM) in a series of 97 cases. Results: Stromal morphological changes, including SC, DR and SA, exhibited significant differences in differential diagnosis. In one indicator, SC had the best sensitivity (90.00%) and accuracy (85.42%), and SA had the best specificity at 88.89 %. In combinations, SC +and SA +were favourable and optimal. The immunoreactivity of GPC3, HSP70 and GS increased significantly in line with the stepwise progression of hepatocarcinogenesis. Conclusions: Stromal histopathology features are useful for diagnosing HGDN, eHCC and small HCC. The immunostaining panel of GPC3, HSP70 and GS can also be supplementary.