In situ localisation of Yersinia enterocolitica by catalysed reported deposition signal amplification. Issue 6 (June 1998)

• Record Type: Journal Article
• Authors: Odinot, P T; Meis, J F; Hoogkamp-Korstanje, J A; Melchers, W J
• Is Part Of: Journal of clinical pathology. Volume 51:Issue 6(1998)
• Page Start: 444
• Page End: 449
• Publication Date: 1998-06
• Subjects: Pathology -- Periodicals; Pathology, Molecular -- Periodicals; 616.0705
• DOI: 10.1136/jcp.51.6.444
• Languages: English
• ISSNs: 0021-9746

Abstract

Abstract : AIM: The sensitive detection of pathogenic Yersinia enterocolitica in paraffin embedded tissue sections by in situ hybridisation (ISH). METHODS: Y enterocolitica infected cell lines, rat spleens, and patient biopsy specimens were used to compare conventional ISH, immune fluorescence assay (IFA) detection, and catalysed reporter deposition (CARD) signal amplification ISH. RESULTS: CARD-ISH was shown to be more sensitive then conventional ISH and had a comparable sensitivity to IFA. In contrast to IFA, CARD-ISH preserved good tissue morphology. CONCLUSIONS: CARD-ISH appeared to be a fast and sensitive ISH method for detecting Y enterocolitica in routinely processed tissue sections. Application of this method allows the combination of routine detection and cellular localisation of the pathogen within the infected tissue.