Possible involvement of IL-12 expression by Epstein-Barr virus in Sjögren syndrome. Issue 11 (1st November 1999)

• Record Type: Journal Article
• Authors: Horiuchi, M; Yamano, S; Inoue, H; Ishii, J; Nagata, Y; Adachi, H; Ono, M; Renard, J N; Mizuno, F; Hayashi, Y; Saito, I
• Is Part Of: Journal of clinical pathology. Volume 52:Issue 11(1999)
• Page Start: 833
• Page End: 837
• Publication Date: 1999-11-01
• Subjects: Pathology -- Periodicals; Pathology, Molecular -- Periodicals; 616.0705
• DOI: 10.1136/jcp.52.11.833
• Languages: English
• ISSNs: 0021-9746

Abstract

Abstract : AIM: To determine the correlation between interleukin 12 (IL-12) expression and Epstein-Barr virus (EBV) in Sjögren syndrome. METHODS: Indirect immunohistochemical technique, enzyme linked immunosorbent assay (ELISA), and immunoblot analysis were used to investigate IL-12 expression by EBV activation, using 13 surgical specimens and four B cell lines. RESULTS: Marked expression of IL-12 was found in the epithelial cells and the infiltrating B cells of salivary gland tissues from patients with Sjögren syndrome (six of 10 cases), but not in those from normal individuals (none of three cases). A striking topographic correlation between IL-12 and EBV was found. In addition, levels of IL-12 production by B cell lines were clearly enhanced by EBV activation in vitro. CONCLUSIONS: IL-12 expression closely reflects the intracellular event of EBV activation in Sjögren syndrome, and may contribute to the T helper cell type 1 (Th1) cytokine overexpression seen in this disease.