Transcription factor NF-κB in a basal metazoan, the sponge, has conserved and unique sequences, activities, and regulation. (March 2020)

• Record Type: Journal Article
• Authors: Williams, Leah M.; Inge, Melissa M.; Mansfield, Katelyn M.; Rasmussen, Anna; Afghani, Jamie; Agrba, Mikhail; Albert, Colleen; Andersson, Cecilia; Babaei, Milad; Babaei, Mohammad; Bagdasaryants, Abigail; Bonilla, Arianna; Browne, Amanda; Carpenter, Sheldon; Chen, Tiffany; Christie, Blake; Cyr, Andrew; Dam, Katie; Dulock, Nicholas; Erdene, Galbadrakh; Esau, Lindsie; Esonwune, Stephanie; Hanchate, Anvita; Huang, Xinli; Jennings, Timothy; Kasabwala, Aarti; Kehoe, Leanne; Kobayashi, Ryan; Lee, Migi; LeVan, Andre; Liu, Yuekun; Murphy, Emily; Nambiar, Avanti; Olive, Meagan; Patel, Devansh; Pavesi, Flaminio; Petty, Christopher A.; Samofalova, Yelena; Sanchez, Selma; Stejskal, Camilla; Tang, Yinian; Yapo, Alia; Cleary, John P.; Yunes, Sarah A.; Siggers, Trevor; Gilmore, Thomas D.;
• Is Part Of: Developmental and comparative immunology. Volume 104(2020)
• Publication Date: 2020-03
• Subjects: NF-kappaB -- Evolution -- Sponge -- Signal transduction -- Immunity -- Amphimedon queenslandica; aa amino acid(s) -- Aq Amphimedon queenslandica -- Ap Aiptasia -- ASW artificial seawater -- Hu human -- IκB IkappaB -- IKK IkappaB kinase -- LPS lipopolysaccharide -- NF-κB nuclear factor kappaB -- Nv Nematostella vectensis -- PBS phosphate-buffered saline; Immunology -- Periodicals; Developmental immunology -- Periodicals; 616.079
• DOI: 10.1016/j.dci.2019.103559
• Languages: English
• ISSNs: 0145-305X

Abstract

Abstract: Herein, we characterize transcription factor NF-κB from the demosponge Amphimedon queenslandica (Aq). Aq–NF–κB is most similar to NF-κB p100/p105 among vertebrate proteins, with an N-terminal DNA-binding domain, a C-terminal Ankyrin (ANK) repeat domain, and a DNA binding-site profile akin to human NF-κB proteins. Like mammalian NF-κB p100, C-terminal truncation allows nuclear translocation of Aq–NF–κB and increases its transcriptional activation activity. Expression of IκB kinases (IKKs) induces proteasome-dependent C-terminal processing of Aq–NF–κB in human cells, and processing requires C-terminal serines in Aq–NF–κB. Unlike NF-κB p100, C-terminal sequences of Aq–NF–κB do not inhibit its DNA-binding activity. Tissue of a black encrusting demosponge contains NF-κB site DNA-binding activity, as well as nuclear and processed NF-κB. Treatment of sponge tissue with LPS increases both DNA-binding activity and processing of NF-κB. A. queenslandica transcriptomes contain homologs to upstream NF-κB pathway components. This is first functional characterization of NF-κB in sponge, the most basal multicellular animal. Highlights: Proteins in the immunity transcription factor NF-κB pathway are present in sponge. Activities and regulation of sponge NF-κB proteins are characterized. LPS treatment of sponge tissue activates NF-κB processing and DNA binding. NF-κB likely has a role in immunity in sponges, the most basal multicellular animals.