Ankylosing spondylitis is associated with the anthrax toxin receptor 2 gene (ANTXR2). Issue 11 (28th August 2014)

• Record Type: Journal Article
• Authors: Karaderi, T; Keidel, S M; Pointon, J J; Appleton, L H; Brown, M A; Evans, D M; Wordsworth, B P
• Is Part Of: Annals of the rheumatic diseases. Volume 73:Issue 11(2014)
• Page Start: 2054
• Page End: 2058
• Publication Date: 2014-08-28
• Subjects: Ankylosing Spondylitis -- Inflammation -- Gene Polymorphism -- Epidemiology -- Spondyloarthritis; Rheumatism -- Periodicals; 616.723005
• DOI: 10.1136/annrheumdis-2014-205643
• Languages: English
• ISSNs: 0003-4967

Abstract

Abstract : Objectives: ANTXR2 variants have been associated with ankylosing spondylitis (AS) in two previous genome-wide association studies (GWAS) (p∼9×10 −8 ). However, a genome-wide significant association (p<5×10 −8 ) was not observed. We conducted a more comprehensive analysis of ANTXR2 in an independent UK sample to confirm and refine this association. Methods: A replication study was carried out with 2978 cases and 8365 controls. Then, these were combined with non-overlapping samples from the two previous GWAS in a meta-analysis. Human leukocyte antigen (HLA)-B27 stratification was also performed to test for ANTXR2 -HLA-B27 interaction. Results: Out of nine single nucleotide polymorphisms (SNP) in the study, five SNPs were nominally associated (p<0.05) with AS in the replication dataset. In the meta-analysis, eight SNPs showed evidence of association, the strongest being with rs12504282 (OR=0.88, p=6.7×10 −9 ). Seven of these SNPs showed evidence for association in the HLA-B27-positive subgroup, but none was associated with HLA-B27-negative AS. However, no statistically significant interaction was detected between HLA-B27 and ANTXR2 variants. Conclusions: ANTXR2 variants are clearly associated with AS. The top SNPs from two previous GWAS (rs4333130 and rs4389526) and this study (rs12504282) are in strong linkage disequilibrium (r 2 ≥0.76). All are located near a putative regulatory region. Further studies are required to clarify the role played by these ANTXR2 variants in AS.