The loss of calretinin expression indicates aganglionosis in Hirschsprung's disease. Issue 7 (25th June 2004)

• Record Type: Journal Article
• Authors: Barshack, I; Fridman, E; Goldberg, I; Chowers, Y; Kopolovic, J
• Is Part Of: Journal of clinical pathology. Volume 57:Issue 7(2004)
• Page Start: 712
• Page End: 716
• Publication Date: 2004-06-25
• Subjects: HD, Hirschsprung's disease -- ICC, interstitial cell of Cajal -- NSE, neurone specific enolase; Hirschsprung's disease -- calretinin -- S100 -- neurone specific enolase -- c-kit; Pathology -- Periodicals; Pathology, Molecular -- Periodicals; 616.0705
• DOI: 10.1136/jcp.2004.016030
• Languages: English
• ISSNs: 0021-9746

Abstract

Abstract : Background: Hirschsprung's disease (HD) is a congenital disorder characterised by the absence of ganglion cells in the large bowel, leading to functional obstruction and colonic dilatation proximal to the affected segment. A subclass of nerve cell bodies in both submucosa and myenteric ganglia of the human gastrointestinal tract were found to show immunopositivity for calretinin, a calcium binding protein, which plays an important role in the organisation and functioning of the central nervous system. Aim: To investigate calretinin immunostaining in ganglionic and aganglionic HD colon specimens, and compare it with staining for S100, neurone specific enolase, and c-kit. Methods: Ten large bowel, full thickness specimens from patients with classic rectosigmoid HD were selected from the pathology repository. In total, 54 paraffin wax blocks—24 from the ganglionic zone, 17 from the aganglionic zone, and 13 from the transitional zone—were processed. Results: Calretinin was not expressed in aganglionic segments of HD and associated nerve fibres, whereas in ganglionic HD segments and in normal colon both ganglion cells and nerve fibres were immunopositive. In addition, c-kit showed an altered distribution in the interstitial cells of Cajal. The transitional zone showed a broad spectrum of histomorphological and immunohistochemical patterns of both calretinin and c-kit expression. Conclusion: The absence of calretinin expression may serve as a diagnostic aid in identifying aganglionic segments in HD.