Lactic acid bacteria secrete metabolites retaining anti-inflammatory properties after intestinal transport. Issue 6 (11th May 2004)

• Record Type: Journal Article
• Authors: Ménard, S; Candalh, C; Bambou, J C; Terpend, K; Cerf-Bensussan, N; Heyman, M
• Is Part Of: Gut. Volume 53:Issue 6(2004)
• Page Start: 821
• Page End: 828
• Publication Date: 2004-05-11
• Subjects: probiotics -- inflammatory cytokines -- intestinal barrier; FCS, fetal calf serum -- IFN, interferon -- IL, interleukin -- LBP, LPS binding protein -- LPS, lipopolysaccharide -- LTA, lipoteichoic acid -- NFκB, nuclear factor κB -- PBMC, peripheral blood mononuclear cells -- SAB, human serum AB -- Bb CM, Bifidobacterium breve conditioned media -- St CM, Streptococcus thermophilus conditioned media -- TGF-β, transforming growth factor β -- TNF-α, tumour necrosis factor α -- TLR, toll-like receptor -- IEC, intestinal epithelial cells; Gastroenterology -- Periodicals; 616.33
• DOI: 10.1136/gut.2003.026252
• Languages: English
• ISSNs: 0017-5749

Abstract

Abstract : Background: Probiotic bacteria have a beneficial effect on intestinal inflammation. In this study, we have examined the effect of lactic acid and commensal Gram positive (+) bacteria conditioned media (CM) on tumour necrosis factor α (TNF-α) release and the mechanisms involved. Methods: Lipopolysaccharide (LPS) induced TNF-α secretion by peripheral blood mononuclear cells or the THP-1 cell line was monitored in the presence or absence of bacteria CM obtained from two probiotic strains, Bifidobacterium breve ( Bb ) and Streptococcus thermophilus ( St ), and three commensal bacterial strains ( Bifidobacterium bifidum, Ruminococcus gnavus, and unidentified Streptococcus ). Bb and St bacteria CM were allowed to cross filter grown intestinal epithelial cell monolayers (HT29-19A) to assess intestinal transport of active bacterial products. These products were characterised and their effect on LPS binding to THP-1 cells and nuclear factor κB (NFκB) activation assessed. Results: Dose dependent inhibition of LPS induced TNF-α secretion was noted for both probiotic bacteria CM (64% and 71% inhibition for Bb and St, respectively) and to a lesser extent commensal bacteria CM (21–32% inhibition). Active products from Bb and St were resistant to digestive enzymes and had a molecular mass <3000 Da. Their inhibitory effect was preserved after transepithelial transport across intestinal cell monolayers, mainly in inflammatory conditions. LPS-FITC binding to THP-1 cells and NFκB activation were significantly inhibited by Bb and St CM. Conclusion: B breve and S thermophilus release metabolites exerting an anti-TNF-α effect capable of crossing the intestinal barrier. Commensal bacteria also display a TNF-α inhibitory capacity but to a lesser extent. These results underline the beneficial effect of commensal bacteria in intestinal homeostasis and may explain the role of some probiotic bacteria in alleviating digestive inflammation.