In vitro effect of cyclosporin A on immunoglobulin production and concanavalin A induced suppression in primary biliary cirrhosis. Issue 3 (March 1986)

• Record Type: Journal Article
• Authors: Al-Aghbar, M N; Alexander, G J; Nouri-Aria, K T; Neuberger, J; Eddleston, A L; Williams, R
• Is Part Of: Gut. Volume 27:Issue 3(1986)
• Page Start: 317
• Page End: 323
• Publication Date: 1986-03
• Subjects: Gastroenterology -- Periodicals; 616.33
• DOI: 10.1136/gut.27.3.317
• Languages: English
• ISSNs: 0017-5749

Abstract

Abstract : The in vitro effect of Cyclosporin A on the regulation of immunoglobulin production was investigated in 16 patients with primary biliary cirrhosis. A significant improvement in concanavalin A induced suppression of IgG and IgM producing cells was observed after prior incubation of mononuclear cells with 300 ng/ml Cyclosporin A for 30 minutes. No effect was seen on spontaneous or pokeweed mitogen induced immunoglobulin production, nor on con A induced suppression if Cyclosporin A was added after 24 hours. Incubation of mononuclear cells with a variable dose of Cyclosporin A showed an effect only at 250-500 ng/ml. Higher and lower doses had no effect. This dose dependent effect of Cyclosporin A is likely to be related to a differential inhibitory effect on T helper and T suppressor cells and may underlie the clinical benefit being observed in current clinical trials.