Production of adiponectin, an anti-inflammatory protein, in mesenteric adipose tissue in Crohn's disease. Issue 6 (11th May 2005)

• Record Type: Journal Article
• Authors: Yamamoto, K; Kiyohara, T; Murayama, Y; Kihara, S; Okamoto, Y; Funahashi, T; Ito, T; Nezu, R; Tsutsui, S; Miyagawa, J-I; Tamura, S; Matsuzawa, Y; Shimomura, I; Shinomura, Y
• Is Part Of: Gut. Volume 54:Issue 6(2005)
• Page Start: 789
• Page End: 796
• Publication Date: 2005-05-11
• Subjects: CD, Crohn's disease -- UC, ulcerative colitis -- TNF-α, tumour necrosis factor-α -- IL, interleukin -- BMI, body mass index -- ELISA, enzyme linked immunosorbent assay -- RT-PCR, reverse transcription-polymerase chain reaction -- cDNA, complementary DNA -- CRP, C reactive protein -- PPARγ, peroxisome proliferator activated receptor γ; Crohn's disease -- fat wrapping -- mesenteric adipocyte -- adiponectin; Gastroenterology -- Periodicals; 616.33
• DOI: 10.1136/gut.2004.046516
• Languages: English
• ISSNs: 0017-5749

Abstract

Abstract : Background and aims: A characteristic feature of Crohn's disease (CD) is mesenteric adipose tissue hypertrophy. Mesenteric adipocytes or specific proteins secreted by them may play a role in the pathogenesis of CD. We recently identified adiponectin as an adipocyte specific protein with anti-inflammatory properties. Here we report on expression of adiponectin in mesenteric adipose tissue of CD patients. Methods and results: Mesenteric adipose tissue specimens were obtained from patients with CD (n = 22), ulcerative colitis (UC) (n = 8) and, for controls, colon carcinoma patients (n = 28) who underwent intestinal resection. Adiponectin concentrations were determined by enzyme linked immunosorbent assay, and adiponectin mRNA levels were determined by real time quantitative reverse transcription-polymerase chain reaction. Tissue concentrations and release of adiponectin were significantly increased in hypertrophied mesenteric adipose tissue of CD patients compared with normal mesenteric adipose tissue of CD patients (p = 0.002, p = 0.040, respectively), UC patients (p = 0.002, p = 0.003), and controls (p<0.0001, p<0.0001). Adiponectin mRNA levels were significantly higher in hypertrophied mesenteric adipose tissue of CD patients than in paired normal mesenteric adipose tissue from the same subjects (p = 0.024). Adiponectin concentrations in hypertrophied mesenteric adipose tissue of CD patients with an internal fistula were significantly lower than those of CD patients without an internal fistula (p = 0.003). Conclusions: Our results suggest that adipocytes in hypertrophied mesenteric adipose tissue produce and secrete significant amounts of adiponectin, which could be involved in the regulation of intestinal inflammation associated with CD.