Direct secretory effect of interleukin-1 via type I receptors in human colonic mucous epithelial cells (HT29-C1.16E). Issue 2 (February 1996)

• Record Type: Journal Article
• Authors: Jarry, A; Vallette, G; Branka, J E; Laboisse, C
• Is Part Of: Gut. Volume 38:Issue 2(1996)
• Page Start: 240
• Page End: 242
• Publication Date: 1996-02
• Subjects: Gastroenterology -- Periodicals; 616.33
• DOI: 10.1136/gut.38.2.240
• Languages: English
• ISSNs: 0017-5749

Abstract

Abstract : The stable differentiated human colonic epithelial cell line, HT29-C1.16E, was used to study the effects of interleukin-1 (IL-1) on mucin exocytosis. The main findings include: (a) IL-1 stimulated a rapid release of mucin from filter grown HT29-C1.16E cells, this effect being dose related; (b) this secretory effect was abolished in the presence of the blocking monoclonal antibody M4 specific for IL-1 receptors type I, showing that IL-1 receptors type I mediated IL-1 action; (c) experiments based on chamber cultures showed that these receptors were located on the basolateral membranes of HT29-C1.16E cells; (d) finally, mRNA for IL-1 receptors type I were detected by reverse transcriptase-polymerase chain reaction in these cells. To extend these findings to the in vivo situation, the rapid stimulatory effect of IL-1 on mucin exocytosis may contribute to the wash out of noxious agents during mucosal inflammation.