Laboratory reference intervals in the assessment of iron status in young children. Issue 1 (31st August 2017)

• Record Type: Journal Article
• Authors: Parkin, Patricia C; Hamid, Jemila; Borkhoff, Cornelia M; Abdullah, Kawsari; Atenafu, Eshetu G; Birken, Catherine S; Maguire, Jonathon L; Azad, Azar; Higgins, Victoria; Adeli, Khosrow
• Is Part Of: BMJ paediatrics open. Volume 1:Issue 1(2017)
• Publication Date: 2017-08-31
• Subjects: reference intervals -- decision limits -- iron deficiency -- haemoglobin -- serum ferritin; Pediatrics -- Periodicals; Children -- Health and hygiene -- Periodicals; 618.920005
• DOI: 10.1136/bmjpo-2017-000074
• Languages: English
• ISSNs: 2399-9772

Abstract

Abstract : Objectives: The primary objective was to establish reference intervals for laboratory tests used to assess iron status in young children using the Clinical and Laboratory Standards Institute guidelines. A secondary objective was to compare the lower limit of the reference interval with the currently recommended cut-off value for haemoglobin and serum ferritin in children 1–3 years of age. Methods: Blood samples were obtained from healthy children recruited during scheduled health supervision visits with their primary care physician. For our primary objective, outliers were removed; age partitions were selected and analysis of variance and pairwise comparisons were made between adjacent partitions; reference intervals and 90% CIs were calculated. For our secondary objective, we determined the proportion of children misclassified using the lower limit reference interval compared with the cut-off value. Results: Samples from 2305 male and 2029 female participants (10 days to 10.6 years) were used to calculate age and sex-specific reference intervals for laboratory tests of iron status. There were statistically significant differences between adjacent age partitions for most analytes. Approximately 10% of children 1–3 years of age were misclassified (underestimated) using the lower limit of the reference intervals rather than the currently recommended cut-off values for haemoglobin and serum ferritin. Implications and relevance: Clinical laboratories may consider adopting published paediatric reference intervals. Reference intervals may misclassify (underestimate) children with iron deficiency as compared with currently recommended cut-off values. Future research on decision limits derived from clinical studies of outcomes is a priority.