Monocyte chemoattractant protein 1 (MCP-1) released from Helicobacter pylori stimulated gastric epithelial cells induces cyclooxygenase 2 expression and activation in T cells. Issue 9 (11th August 2003)

• Record Type: Journal Article
• Authors: Futagami, S; Hiratsuka, T; Tatsuguchi, A; Suzuki, K; Kusunoki, M; Shinji, Y; Shinoki, K; Iizumi, T; Akamatsu, T; Nishigaki, H; Wada, K; Miyake, K; Gudis, K; Tsukui, T; Sakamoto, C
• Is Part Of: Gut. Volume 52:Issue 9(2003)
• Page Start: 1257
• Page End: 1264
• Publication Date: 2003-08-11
• Subjects: Helicobacter pylori -- T cell -- cyclooxygenase -- COX-2 inhibitor -- mucosal immunity; MCP-1, monocyte chemoattractant protein 1 -- COX, cyclooxygenase -- IL, interleukin -- IFN-γ interferon γ -- PG, prostaglandin -- HPWEP, Helicobacter pylori water extract protein -- FCS, fetal calf serum -- NFκB, nuclear factor κB -- MIP, macrophage inflammatory protein -- ELISA, enzyme linked immunosorbent assay -- RT-PCR, reverse transcription-polymerase chain reaction -- TBS, Tris buffered saline -- LPS, lipopolysaccharide -- Th, T helper; Gastroenterology -- Periodicals; 616.33
• DOI: 10.1136/gut.52.9.1257
• Languages: English
• ISSNs: 0017-5749

Abstract

Abstract : Background and aims: To clarify the interaction between gastric epithelial and mucosal T cells, we examined the role of cytokines released from epithelial cells in response to Helicobacter pylori water extract protein (HPWEP) in regulating T cell cyclooxygenase 2 (COX-2) expression and activation. Methods: Media from MKN-28 cells incubated with HPWEP for 48 hours were added to Jurkat T cells and human peripheral T cells. C–C and CXC chemokine concentrations in MKN-28 cell media, and COX-2 expression, interferon γ (IFN-γ), and interleukin (IL)-4 secretions in T cells were determined by western blot analysis and ELISA methods. Distributions of COX-2 positive T cells and monocyte chemoattractant protein 1 (MCP-1) in tissue specimens with H pylori associated gastritis were determined as single or double labelling by immunohistochemistry. Results: MCP-1, IL-7, IL-8, and RANTES were detected in media from MKN-28 cells incubated with HPWEP . Media as a whole, and MCP-1 alone, stimulated COX-2 expression and peripheral T cell proliferation. Anti-MCP-1 antibody inhibited media stimulated COX-2 mRNA expression in Jurkat T cells. Media stimulated IFN-γ but not IL-4 secretion from peripheral T cells, while MCP-1 stimulated IL-4 but not IFN-γ secretion. Both stimulated cytokine release, and peripheral T cell proliferation was partially inhibited by NS-398, a specific COX-2 inhibitor. In mucosa with gastritis, COX-2 was expressed in T cells and MCP-1 was localised mainly in epithelial and mononuclear cells. MCP-1 levels and the intensity of COX-2 expression in tissue samples were closely related. Conclusions: Cytokines such as MCP-1, released from gastric epithelial cells in response to HPWEP, seem to modulate T cell immune responses, at least in part via COX-2 expression.