LncRNA SENCR promotes cell proliferation and progression in non-small-cell lung cancer cells via sponging miR-1-3p. Issue 14 (18th July 2021)

• Record Type: Journal Article
• Authors: Cheng, Ruirui; Zhang, Guowei; Bai, Yong; Zhang, Furui; Zhang, Guojun
• Is Part Of: Cell cycle. Volume 20:Issue 14(2021)
• Page Start: 1402
• Page End: 1414
• Publication Date: 2021-07-18
• Subjects: LncSENCR -- miR-1-3p -- cdk4 -- cdk6 -- non-small cell lung cancer; Cell cycle -- Periodicals; 571.84377
• DOI: 10.1080/15384101.2021.1924958
• Languages: English
• ISSNs: 1538-4101

Abstract

ABSTRACT: Studies have found that lncRNA SENCR (lncSENCR) is closely related to the progress of human cancer. This study aimed to investigate the biological and clinical roles of lncSENCR in non-small cell lung cancer (NSCLC). RT-qPCR was used to detect the expression of lncSENCR and miR-1-3p in NSCLC. CCK-8 assay, Transwell assay and EDU staining were used to detect the effects of lncSENCR and miR-1-3p on the proliferation, invasion and migration of NSCLC cells. Target gene prediction and screening, luciferase reporter assay were used to validate downstream target genes of lncSENCR and miR-1-3p. Western blotting was used to detect the protein expression levels of CDK4 and CDK6. The tumor changes in mice were detected by in vivo experiments in nude mice. The expression levels of lncSENCR were increased in NSCLC, while the expression levels of miR-1-3p were reduced. Knockdown of lncSENCR inhibited the growth and metastasis of NSCLC. LncSENCR was found to regulate tumor growth through miR-1-3p. Knockdown of miR-1-3p reversed the inhibitory effect of sh-SENCR on the proliferation of NSCLC cells. Furthermore, lncSENCR regulated the expression of CDK4 and CDK6 by acting as a cavern of miR-1-3p in NSCLC. LncSENCR promotes cell proliferation and progression of NSCLC by regulating the expression of miR-1-3p, and CDK4 and CDK6 may be involved in the underlying mechanism of lncSENCR in NSCLC. Abbreviation: NSCLC: Non-small cell lung cancer.