Study on the binding of sulfaclozine sodium monohydrate with bovine and human serum albumins using multi-spectroscopy and molecular docking. Issue 13 (2nd September 2021)

• Record Type: Journal Article
• Authors: Li, Xu; Sun, Xiaoyue; Zhao, Rui; Shao, Di; Bi, Shuyun
• Is Part Of: Journal of biomolecular structure & dynamics. Volume 39:Issue 13(2021)
• Page Start: 4835
• Page End: 4844
• Publication Date: 2021-09-02
• Subjects: Sulfaclozine -- sodium monohydrate -- serum albumins -- multi-spectroscopy -- molecular docking; Biomolecules -- Periodicals; Molecular structure -- Periodicals; Molecular Biology -- Periodicals; Biomechanics -- Periodicals; 572
• DOI: 10.1080/07391102.2020.1780945
• Languages: English
• ISSNs: 0739-1102

Abstract

Abstract: The interactions of sulfaclozine sodium monohydrate (SSM) with bovine and human serum albumins (BSA and HSA) were studied by multi-spectroscopy and molecular docking technique. Stern–Volmer analysis and fluorescence lifetime measurements suggested the quenching processes were static. According to the Fluorescence resonance energy transfer (FRET) theory, the binding distances were obtained indicating SSM interacted with BSA/HSA along with non-radiation energy conversion. Electrostatic attraction was the main force in keeping the stability of the compound based on thermodynamic parameters. Circular dichroism (CD), synchronous fluorescence and Fourier Transform infrared (FT-IR) spectra embodied the secondary structures of serum albumins were transformed by SSM. The site marker competitive and molecular docking measurements testified SSM bound to BSA/HSA at site I. In conclusion, the secondary structures of BSA/HSA were changed by SSM in the static fluorescence quenching processes with the non-radiation energy conversion. The binding sites were all located at site I and electrostatic attraction was the main force for the new compound. Communicated by Ramaswamy H. Sarma