Efficiency enhancement of a new cellulase cocktail at low enzyme loading for high solid digestion of alkali catalyzed atmospheric glycerol organosolvent pre-treated sugarcane bagasse. (October 2021)

• Record Type: Journal Article
• Authors: Nwamba, Marknoah Chinenye; Song, Guojie; Sun, Fubao; Mukasekuru, Marie Rose; Ren, Hongyan; Zhang, Qing; Cao, Tishuang; Wang, Huaming; Sun, Haiyan; Hong, Jiong
• Is Part Of: Bioresource technology. Volume 338(2021)
• Publication Date: 2021-10
• Subjects: Lignocellulosic biomass -- Organosolv pretreatment -- Enzymatic saccharification -- Accessory enzymes and additives -- Cellulase cocktail; Biomass -- Periodicals; Biomass energy -- Periodicals; Bioremediation -- Periodicals; Agricultural wastes -- Periodicals; Factory and trade waste -- Periodicals; Organic wastes -- Periodicals; Bioénergie -- Périodiques; Déchets agricoles -- Périodiques; Déchets industriels -- Périodiques; Déchets organiques -- Périodiques; Déchets (Combustible) -- Périodiques; 662.88
• DOI: 10.1016/j.biortech.2021.125505
• Languages: English
• ISSNs: 0960-8524

Abstract

Graphical abstract: Highlights: This study evaluated the hydrolytic efficiency of a new enzyme cocktail. A high-solids content (20%) hydrolysis system was developed via fed-batch mode. Enzyme dosage was reduced by augmentation with additives and accessory enzymes. 2 mg protein/ g biomass LT4 loading plus optimized supplements yielded 83% glucose. Abstract: The acquisition during biomass saccharification of elevated levels of fermentable sugars with lower cellulase concentration is central to ensuring an economically viable and industrially relevant hydrolytic process. Thus, using a new cellulase preparation (LT4) at low cellulase loading (2 mg protein/g dried substrate), this study assessed the possible boosting effect of integrating accessory enzymes and additives on high-solids hydrolysis of sugarcane bagasse via fed-batch feeding. Hydrolysis which commenced with initial 8% solids loading and subsequent substrate feeding of 4% solids at 6 h, 18 h, and 24 h respectively, proved optimal for the 20% high-solids saccharification producing 158 g/L total sugars and 83% glucose yield after 72 h with the combined optimized additives and accessory enzymes. The results obtained indicate that the integration of accessory enzymes and additives offers a benignant approach to minimizing the enzyme load and cost of high solids saccharification of lignocellulosic heteropolymers while also boosting enzyme hydrolytic performance.