The usefulness of a novel fully automated PCR-based Idylla test for detection of the BRAF V600E mutation in thyroid tissue: comparison with PNA-clamping PCR, real-time PCR and pyrosequencing. Issue 3 (19th August 2016)

• Record Type: Journal Article
• Authors: Yeo, Min-Kyung; Jung, Min-Kyu; Lee, Su-Yel; Lee, Yong-Moon; Hur, Gang Min; Kim, Jin-Man
• Is Part Of: Journal of clinical pathology. Volume 70:Issue 3(2017)
• Page Start: 260
• Page End: 265
• Publication Date: 2016-08-19
• Subjects: THYROID -- MOLECULAR PATHOLOGY -- ENDOCRINE PATHOLOGY; Pathology -- Periodicals; Pathology, Molecular -- Periodicals; 616.0705
• DOI: 10.1136/jclinpath-2016-204025
• Languages: English
• ISSNs: 0021-9746

Abstract

Abstract : Introduction: The BRAF V600E mutation is the most common genetic event in papillary thyroid carcinoma (PTC). The BRAF V600E mutational status has a significant diagnostic and prognostic role in PTC since it can be detected in 32%–87% of PTC by various molecular methods. Aim(s): A novel, fully automated real-time PCR-based Idylla test is assessed to detect the BRAF mutation in formalin-fixed paraffin-embedded (FFPE) thyroid samples. Methods: 99 PTC and 11 nodular hyperplasia FFPE thyroid tissues are evaluated for the BRAF V600E mutation by the Idylla tests and compared with peptide nucleic acid-clamping PCR, real-time PCR and pyrosequencing. Results: The sensitivity and specificity of the Idylla test to detect BRAF V600E are 98.8% and 100%, which is superior to real-time PCR and pyrosequencing. The concordance between Idylla and true positive is highest at 0.974. Conclusions: This study validates that the Idylla test is a sensitive and specific method to detect BRAF V600E in FFPE thyroid tissues. A simple, quick and easy to handle Idylla test is a useful and reliable molecular technique to evaluate BRAF mutations.