Direct polymerase chain reaction amplification of formalin-fixed, paraffin-wax-embedded tissue after automated sequential laser microdissection and pressure catapulting. Issue 2 (30th January 2007)

• Record Type: Journal Article
• Authors: O'Kane, S L; Garimella, V; Sivarajasingham, N; Drew, P J; Cawkwell, L
• Is Part Of: Journal of clinical pathology. Volume 60:Issue 2(2007)
• Page Start: 216
• Page End: 217
• Publication Date: 2007-01-30
• Subjects: dPCR, direct polymerase chain reaction -- FFPE, formalin-fixed, paraffin-wax-embedded -- LMPC, laser microdissection and pressure catapulting -- PCR, polymerase chain reaction -- PEN, polyethylene naphtahalate -- sLMPC, sequential laser microdissection and pressure catapulting; Pathology -- Periodicals; Pathology, Molecular -- Periodicals; 616.0705
• DOI: 10.1136/jcp.2006.037275
• Languages: English
• ISSNs: 0021-9746

Abstract

Abstract : A robust method to facilitate rapid laser microdissection and pressure catapulting (LMPC) coupled with direct polymerase chain reaction (dPCR) to eliminate the need for extraction of DNA before a PCR-based assay is described. This sequential LMPC–dPCR method is rapid and decreases the number of processing steps, reducing the chance of tissue loss and contamination.