C-erbB-2 overexpression in the dysplasia/carcinoma sequence of Barrett's oesophagus. Issue 2 (February 1995)

• Record Type: Journal Article
• Authors: Hardwick, R H; Shepherd, N A; Moorghen, M; Newcomb, P V; Alderson, D
• Is Part Of: Journal of clinical pathology. Volume 48:Issue 2(1995)
• Page Start: 129
• Page End: 132
• Publication Date: 1995-02
• Subjects: Pathology -- Periodicals; Pathology, Molecular -- Periodicals; 616.0705
• DOI: 10.1136/jcp.48.2.129
• Languages: English
• ISSNs: 0021-9746

Abstract

Abstract : AIMS--To investigate overexpression of the oncoprotein c-erbB-2 in the dysplasia/carcinoma sequence of Barrett's columnar-lined oesophagus (CLO). METHODS--Immunohistochemical staining was performed using the monoclonal antibody NCL-CB-11 on formalin fixed tissue from 31 cases of Barrett's carcinoma, 20 cases of cancer associated dysplastic CLO, seven cases of dysplastic CLO without cancer, and 20 cases of non-dysplastic CLO. Membranous staining was regarded as positive for c-erbB-2 overexpression; cytoplasmic staining was recorded separately as its significance is uncertain. RESULTS--Membranous c-erbB-2 overexpression was observed in eight of 31 (26%) carcinomas and in none of the cases of dysplastic CLO. Variable cytoplasmic staining was seen in four of 31 (13%) tumours and seven of 27 (26%) cases of dysplastic CLO. No staining was observed in non-dysplastic CLO. CONCLUSIONS--C-erbB-2 overexpression is a relatively late event in the development of some Barrett's carcinomas and is unlikely to be involved in the early stages of neoplastic transformation of CLO.