Dehydrogenase enzyme cytochemistry of unfixed leucocytes. Issue 6 (September 1970)

• Record Type: Journal Article
• Authors: Stuart, John; Simpson, Jennifer S.
• Is Part Of: Journal of clinical pathology. Volume 23:Issue 6(1970)
• Page Start: 517
• Page End: 521
• Publication Date: 1970-09
• Subjects: Pathology -- Periodicals; Pathology, Molecular -- Periodicals; 616.0705
• DOI: 10.1136/jcp.23.6.517
• Languages: English
• ISSNs: 0021-9746

Abstract

Abstract : An inert, synthetic, sucrose polymer (Ficoll) has been found to have a protective effect on leucocytes during incubation for cytochemical reactions at 37°C. This permits enzyme cytochemistry to be performed on unfixed bone marrow and peripheral blood smears, and on leucocytes cultured in vitro, thus avoiding enzyme inhibition by the use of a fixative. The technique is suitable for the demonstration of both cytoplasmic and intramitochondrial dehydrogenase enzyme activity.