The reliability of methicillin sensitivity tests on four culture media. Issue 5 (May 1974)

• Record Type: Journal Article
• Authors: Brown, D. F. J.; Kothari, D.
• Is Part Of: Journal of clinical pathology. Volume 27:Issue 5(1974)
• Page Start: 420
• Page End: 426
• Publication Date: 1974-05
• Subjects: Pathology -- Periodicals; Pathology, Molecular -- Periodicals; 616.0705
• DOI: 10.1136/jcp.27.5.420
• Languages: English
• ISSNs: 0021-9746

Abstract

Abstract : The influence of culture medium, incubation temperature, and antibiotic content on the reliability of methicillin disc-sensitivity tests on strains of Staphylococcus aureus was evaluated. Tests were performed with an inoculum giving semiconfluent growth of colonies on four media, with 5-μg and 10-μg methicillin discs at incubation temperatures of 30, 35, and 37°C. With Sensitest agar and Mueller-Hinton agar at 35°C or below, methicillin resistance was reliably detected. On DST agar and Wellcotest agar discrimination between sensitive and resistant strains was possible only at 30°C. On Wellcotest agar and DST agar it was not possible to detect methicillin resistance in one resistant strain at any temperature. For sensitive and resistant strains, the use of a 5-μg disc allowed greater differentiation than the 10-μg disc.