Identifying the Genotypes of Hepatitis B Virus (HBV) with DNA Origami Label. Issue 6 (28th December 2017)

• Record Type: Journal Article
• Authors: Liu, Ke; Pan, Dun; Wen, Yanqin; Zhang, Honglu; Chao, Jie; Wang, Lihua; Song, Shiping; Fan, Chunhai; Shi, Yongyong
• Is Part Of: Small. Volume 14:Issue 6(2018)
• Page Start: n/a
• Page End: n/a
• Publication Date: 2017-12-28
• Subjects: atomic force microscope (AFM) -- DNA origami -- genotype -- HBV; Nanotechnology -- Periodicals; Nanoparticles -- Periodicals; Microtechnology -- Periodicals; 620.5
• DOI: 10.1002/smll.201701718
• Languages: English
• ISSNs: 1613-6810

Abstract

Abstract: The hepatitis B virus (HBV) genotyping may profoundly affect the accurate diagnosis and antiviral treatment of viral hepatitis. Existing genotyping methods such as serological, immunological, or molecular testing are still suffered from substandard specificity and low sensitivity in laboratory or clinical application. In a previous study, a set of high‐efficiency hybridizable DNA origami‐based shape ID probes to target the templates through which genetic variation could be determined in an ultrahigh resolution of atomic force microscopy (AFM) nanomechanical imaging are established. Here, as a further confirmatory research to explore the sensitivity and applicability of this assay, differentially predesigned DNA origami shape ID probes are also developed for precisely HBV genotyping. Through the specific identification of visualized DNA origami nanostructure with clinical HBV DNA samples, the genetic variation information of genotypes can be directly identified under AFM. As a proof‐of‐concept, five genotype B and six genotype C are detected in 11 HBV‐infected patients' blood DNA samples of Han Chinese population in the single‐blinded test. The AFM image‐based DNA origami shape ID genotyping approach shows high specificity and sensitivity, which could be promising for virus infection diagnosis and precision medicine in the future. Abstract : Distinctive DNA origami shape ID probes are developed to precisely identify hepatitis B virus genotypes by targeting clinical virus DNA samples through high‐resolution atomic force microscopy imaging. This approach exhibits high specificity and sensitivity, holding great promise of early virus infection diagnosis and precision clinical medicine translation in the future.