Development and application of a high throughput assay system for the detection of Rieske dioxygenase activity. Issue 4 (13th January 2021)

• Record Type: Journal Article
• Authors: Preston-Herrera, Cristina; Jackson, Aaron S.; Bachmann, Brian O.; Froese, Jordan T.
• Is Part Of: Organic & biomolecular chemistry. Volume 19:Issue 4(2020)
• Page Start: 775
• Page End: 784
• Publication Date: 2021-01-13
• Subjects: Chemistry, Organic -- Periodicals; Bioorganic chemistry -- Periodicals; Chemistry, Physical organic -- Periodicals; 547
• DOI: 10.1039/d0ob02412k
• Languages: English
• ISSNs: 1477-0520

Abstract

Abstract : A new periodate-based reactive assay system enables the rapid evaluation of the cis -dihydroxylation activity of Rieske dioxygenase enzymes. Abstract : Herein we report the development of a new periodate-based reactive assay system for the fluorescent detection of the cis -diol metabolites produced by Rieske dioxygenases. This sensitive and diastereoselective assay system successfully evaluates the substrate scope of Rieske dioxygenases and determines the relative activity of a rationally designed Rieske dioxygenase variant library. The high throughput capacity of the assay system enables rapid and efficient substrate scope investigations and screening of large dioxygenase variant libraries.