Association of Interleukin 16 With the Development of Ovarian Tumor and Tumor-Associated Neoangiogenesis in Laying Hen Model of Spontaneous Ovarian Cancer. Issue 2 (1st February 2012)

• Record Type: Journal Article
• Authors: Yellapa, Aparna; Bahr, Janice M.; Bitterman, Pincas; Abramowicz, Jacques S.; Edassery, Seby L.; Penumatsa, Krishna; Basu, Sanjib; Rotmensch, Jacob; Barua, Animesh
• Is Part Of: International journal of gynecological cancer. Volume 22:Issue 2(2012)
• Page Start: 199
• Page End: 207
• Publication Date: 2012-02-01
• Subjects: Interleukin 16 -- Ovarian tumor -- Tumor-associated neoangiogenesis -- Ovarian cancer; Generative organs, Female -- Cancer -- Periodicals; 616.99465
• DOI: 10.1097/IGC.0b013e318236a27b
• Languages: English
• ISSNs: 1048-891X

Abstract

Abstract : Objective: Tumor-associated neoangiogenesis (TAN) is an early event in ovarian tumor development. Interleukin 16 (IL-16) is a proangiogenic cytokine that stimulates production of neoangiogenic factors. The goal of this study was to determine the association of IL-16 with tumor development and ovarian TAN in laying hens, an animal model of spontaneous ovarian cancer (OVCA). Methods: Sera and ovarian tissues from 3-year-old laying hens were collected and processed for histopathologic, immunoassay, immunohistochemistry, immunoblotting, and molecular biological studies to determine the tissue expression and serum levels of IL-16. Samples were divided into 3 groups based on the diagnosis of the histopathologic ovarian tissue examination, namely, normal (healthy control, n = 81), early (n = 23 including 11 with microscopic OVCA), and late stages (n = 16) of OVCA. Results: Serum levels of IL-16 were significantly higher in hens with microscopic, early, and late stages of OVCA than normal hens ( P < 0.0001). The frequencies of IL-16 + cells in tumor-bearing ovaries were significantly higher than normal hens ( P < 0.05). The expression of IL-16 protein and mRNA were stronger in tumor-bearing ovaries than normal ovaries. In addition to ovarian stroma, IL-16 was also expressed by the epithelial cells of the tumor in OVCA hens. Differences in serum levels and ovarian IL-16 expression were not significant among different histological subtypes of OVCA including serous, endometrioid, and mucinous. Similar to the serum levels and ovarian expression of IL-16, the densities of neoangiogenic microvessels were significantly higher in hens with tumor-bearing ovaries than normal hens. Conclusions: The results of the study suggest that changes in serum levels of IL-16 are associated with tumor development and TAN. Thus, serum IL-16 levels may be an indicator of ovarian TAN at the early stage of OVCA.