Epidermal growth factor receptor determination on tumour imprints. Issue 2 (February 1993)

• Record Type: Journal Article
• Authors: Hastrich, D J; Dunn, J M; Newcomb, P; Hall, P; Rooney, N; Nicholson, S; Farndon, J R
• Is Part Of: Journal of clinical pathology. Volume 46:Issue 2(1993)
• Page Start: 166
• Page End: 168
• Publication Date: 1993-02
• Subjects: Pathology -- Periodicals; Pathology, Molecular -- Periodicals; 616.0705
• DOI: 10.1136/jcp.46.2.166
• Languages: English
• ISSNs: 0021-9746

Abstract

Abstract : Epidermal growth factor receptor (EGFR) is a sensitive prognostic indicator in women with breast cancer. A new technique for the detection of EGFR on breast cancer imprints is described and evaluated in relation to immunohistochemistry on frozen sections. The standard streptavidin-biotin method using the R1 antibody on frozen sections was modified for use on tumour imprints. In the first group (group 1 imprints) 43 tumours were assessed by frozen section and imprint staining. The group 1 imprint method had a sensitivity of 50% and a specificity of 89% compared with the frozen sections. Further modifications were made for the following 32 tumours (group 2) and this method had a much improved sensitivity of 94% while specificity was preserved. This method is simple and could be performed in any hospital histopathology department.