Annexin IV is Differentially Expressed in Clear Cell Carcinoma of the Ovary. Issue 9 (1st November 2009)

• Record Type: Journal Article
• Authors: Miao, Yi; Cai, Bin; Liu, Ling; Yang, Yixia; Wan, Xiaoping
• Is Part Of: International journal of gynecological cancer. Volume 19:Issue 9(2009)
• Page Start: 1545
• Page End: 1549
• Publication Date: 2009-11-01
• Subjects: Clear cell carcinoma of the ovary -- Gene expression profiling -- Annexin IV; Generative organs, Female -- Cancer -- Periodicals; 616.99465
• DOI: 10.1111/IGC.0b013e3181a844c8
• Languages: English
• ISSNs: 1048-891X

Abstract

Abstract : Objective: To investigate the genes that were differentially expressed between clear cell carcinoma (CCC) and serous carcinoma (SAC) of the ovary with complementary DNA microarray. Methods: Complementary DNA microarray was carried out in 8 CCCs and 8 SACs of the ovary. Differentially expressed genes were identified and verified by real-time polymerase chain reaction. The expression of the protein was also verified with immunohistochemistry and Western blot in cells and tissues of ovarian CCC. Results: Comparison of the gene expression profiling identified 21 genes with more than 2-fold different expression between CCC and SAC of the ovary. The up-regulated and down-regulated genes were 9 and 12, respectively. The verification of Annexin IV in the cell line and tissues was in accordance with the result of the microarray. Conclusions: The complementary DNA microarray technique is a feasible way to explore the difference of the gene expression profiling between the 2 types of ovarian carcinoma. The overexpression of Annexin IV may be an ovarian CCC-specific molecular marker. Abbreviations: CCC- clear cell carcinoma, SAC- serous carcinoma, PCR- polymerase chain reaction, RT-PCR- reverse transcriptase PCR, ABCF2- ATP-binding cassette, sub-family F- member 2, HNF-1b- hepatocyte nuclear factor 1β