A new detection method for the K variant of butyrylcholinesterase based on PCR primer introduced restriction analysis (PCR-PIRA). Issue 7 (July 1994)

• Record Type: Journal Article
• Authors: Shibuta, K; Abe, M; Suzuki, T
• Is Part Of: Journal of medical genetics. Volume 31:Issue 7(1994)
• Page Start: 576
• Page End: 579
• Publication Date: 1994-07
• Subjects: Medical genetics -- Periodicals; 616.042
• DOI: 10.1136/jmg.31.7.576
• Languages: English
• ISSNs: 1468-6244

Abstract

Abstract : The K variant of human butyrylcholinesterase is caused by a G/A transition in the butyrylcholinesterase gene, which neither creates nor destroys any restriction site. In an attempt to detect the K variant both simply and rapidly, we developed a two step method of "PCR primer introduced restriction analysis" (PCR-PIRA). The first step was used to introduce a new Fun4HI site into the normal allele for a screening test, while the second step was performed to create a new MaeIII site on the variant allele for a specific test. This method thus enabled us to distinguish clearly the K variant from the normal allele, and also showed that the frequency of the K variant allele is 0.164 in the Japanese population.