Anti-HIV antibodies in the CSF of AIDS patients: a serological and immunoblotting study. Issue 8 (August 1988)

• Record Type: Journal Article
• Authors: Bukasa, K S; Sindic, C J; Bodeus, M; Burtonboy, G; Laterre, C; Sonnet, J
• Is Part Of: Journal of neurology, neurosurgery and psychiatry. Volume 51:Issue 8(1988)
• Page Start: 1063
• Page End: 1068
• Publication Date: 1988-08
• Subjects: Neurology -- Periodicals; Nervous system -- Surgery -- Periodicals; Psychiatry -- Periodicals; 616.8
• DOI: 10.1136/jnnp.51.8.1063
• Languages: English
• ISSNs: 0022-3050

Abstract

Abstract : CSF and serum samples from 16 AIDS patients were tested for the presence of anti-HIV antibodies either by classical serological methods or by an immunoblot technique based on agarose gel isoelectric focusing and transfer of the specific IgG antibodies onto HIV antigens-loaded nitrocellulose sheets. This method enabled the demonstration of an intrathecal synthesis of anti-HIV oligoclonal IgG antibodies, often superimposed on diffuse polyclonal production, in 14 patients. The two negative cases were devoid of neurological signs or symptoms. However, two patients classified in stage II of the disease (asymptomatic infection) displayed an intrathecal synthesis of anti-HIV antibodies.