AB0019 Effect of extracellular sodium concentration in byglican and decorin synthesis by human chondrocytes. (1st June 2001)

• Record Type: Journal Article
• Authors: Ferraz-Amaro, IA; Trujillo, E; Arteaga, MF; Cozar, I; Avila, J; Bustabad, S; Gonzalez, T; Martin-Vasallo, P
• Is Part Of: Annals of the rheumatic diseases. Volume 60(2001)Supplement 1
• Page Start: A360
• Page End: A361
• Publication Date: 2001-06-01
• Subjects: Rheumatism -- Periodicals; 616.723005
• DOI: 10.1136/annrheumdis-2001.913
• Languages: English
• ISSNs: 0003-4967

Abstract

Abstract : Background: An altered chondrocytes phenotipic expression of biglycan and decorin is an early event in osteoarthritis (OA). Changes in extracellular matrix sodium concentration ([Na+]) occurs in OA. Previous results suggested that chondrocytes are sensitive to changes in their extracellular [Na+] environment and respond with variations in the matrix synthesis. Objectives: Here we have studied the influence of extracellular [Na+] on the metabolism of small proteoglycans, biglycan and decorin, in a human chondrocyte-like cell line, C20/A4. Methods: Extracellular deposition of biglycan and decorin was analysed in C20A4 cells cultured: 1) inmunohistochemically using specific antibodies against biglycan and decorin and secondary IFTC conjugated antibodies; and 2) in SDS-PAGE electrophoresis and blotting using the corresponding antibodies. Results: Fluorescence inmunohistochemically shows specific extracellular expression of biglycan and decorin in chondrocytes cultured in estandard [Na+] (154 nm) and western blotting intensities of these small proteoglycans varied with [Na+]. Conclusion: This study demostrates that biglycan and decorin are expressed in the human chondrocyte cell line C20A4. We are now searching if [Na+] variations is able to produce cualitative or cuantitative alterations in the matrix synthesis as it have been found in osteoarthritis. -Supported by FIS grant 99/0695-