Immune monitoring technology primer: immunosequencing. Issue 1 (25th June 2015)

• Record Type: Journal Article
• Authors: Kirsch, Ilan
• Is Part Of: Journal for immunotherapy of cancer. Volume 3:Issue 1(2015)
• Publication Date: 2015-06-25
• Subjects: Immune Receptor -- CDR3 Sequence -- Single Cell Isolation -- Primary Nucleotide Sequence -- Correct Sequencing Error; Cancer -- Immunotherapy -- Periodicals; Cancer -- Immunological aspects -- Periodicals; Tumors -- Immunological aspects -- Periodicals; Immunotherapy -- Periodicals; 616.99406105
• DOI: 10.1186/s40425-015-0076-y
• Languages: English
• ISSNs: 2051-1426

Abstract

Abstract : Background: Profiling of the immune receptor repertoire is becoming increasingly relevant to the understanding and clinical management of cancer, autoimmunity, aging, and infectious disease. Findings: A platform technology is described that provides comprehensive immune receptor profiling. Conclusion: Immunosequencing is a platform technology that allows the enumeration, specification and quantification of each and every B-and/or T-cell in any biologic sample of interest. It is based on bias-controlled multiplex PCR and high throughput sequencing and is highly accurate, standardized, and sensitive.