Evaluation of three selective chromogenic media, CHROMagar ESBL, CHROMagar CTX-M and CHROMagar KPC, for the detection of Klebsiella pneumoniae producing OXA-48 carbapenemase. Issue 4 (2nd January 2013)

• Record Type: Journal Article
• Authors: Hornsey, Michael; Phee, Lynette; Woodford, Neil; Turton, Jane; Meunier, Daniele; Thomas, Claire; Wareham, David W
• Is Part Of: Journal of clinical pathology. Volume 66:Issue 4(2013)
• Page Start: 348
• Page End: 350
• Publication Date: 2013-01-02
• Subjects: Antimicrobial Resistance -- Bacteriology -- Methodology; Pathology -- Periodicals; Pathology, Molecular -- Periodicals; 616.0705
• DOI: 10.1136/jclinpath-2012-201234
• Languages: English
• ISSNs: 0021-9746

Abstract

Abstract : Three selective chromogenic culture media (CHROMagars ESBL, CTX-M and KPC) were evaluated for their ability to support the growth of nine Klebsiella pneumoniae isolates producing OXA-48 carbapenemase in combination with other β-lactamases. CHROMagar ESBL and CHROMagar KPC were the most sensitive media, supporting growth of all isolates with a detection limit as low as < 100 CFU/ml. Five isolates failed to grow on CHROMagar CTX-M, and five were recovered on CHROMagar KPC only at counts > 10 6 CFU/ml. Both CHROMagar ESBL and CHROMagar KPC may be useful for enhanced isolation of K pneumoniae producing OXA-48-like carbapenemases.