Comparison of a modified microagglutination technique for HBsAg with the standard technique and a new RIA system. Issue 12 (December 1981)

• Record Type: Journal Article
• Authors: Supran, E M; Gardner, P S; Cayzer, I; Court, G; Chalmers, S
• Is Part Of: Journal of clinical pathology. Volume 34:Issue 12(1981)
• Page Start: 1396
• Page End: 1399
• Publication Date: 1981-12
• Subjects: Pathology -- Periodicals; Pathology, Molecular -- Periodicals; 616.0705
• DOI: 10.1136/jcp.34.12.1396
• Languages: English
• ISSNs: 0021-9746

Abstract

Abstract : A further modification of the standard RPHA technique (Hepatest, Wellcome Reagents) for the detection of HBsAg is described. This modification does not require a centrifugation step which is required by the other modifications that have been described previously and consequently takes a little longer to perform. It does, however, retain the advantages of increased sensitivity and decreased costs which are also features of the other modifications. A series of 939 routine clinical specimens were used to evaluate the method described and to evaluate a new RIA kit for the detection of HBsAg (Hepatube, Wellcome Reagents). Of 53 specimens found to be positive for HBsAg by RIA, 50 (94% were detected by the modified Hepatest RPHA as opposed to 47 (89%) by the standard technique.