Herpes simplex virus type 1 persistence and latency in cultured rabbit corneal epithelial cells, keratocytes, and endothelial cells. Issue 9 (September 1986)

• Record Type: Journal Article
• Authors: Cook, S D; Brown, S M
• Is Part Of: British journal of ophthalmology. Volume 70:Issue 9(1986)
• Page Start: 642
• Page End: 650
• Publication Date: 1986-09
• Subjects: Ophthalmology -- Periodicals; 617.7
• DOI: 10.1136/bjo.70.9.642
• Languages: English
• ISSNs: 0007-1161

Abstract

Abstract : Cell cultures of rabbit corneal epithelium, keratocytes, and endothelium were used to determine the lytic cycle of herpes simplex virus type 1. Viral growth was fastest in epithelial cells. A novel HSV-1 in-vitro latency system was established in the three distinct cell types. Cell cultures were inoculated at low multiplicities of infection with HSV-1. Temperature manipulation alone was used to induce and reactivate latent HSV-1 infections. The presence of cellular stress proteins was demonstrated at supraoptimal temperatures. All cell types were capable of maintaining latent viral infections under these conditions. Viral persistence was present in 20% of epithelial cell cultures at supraoptimal temperatures, but not in keratocyte cultures or endothelial cell cultures.