S antigen specific effector T cell activation detected by cytokine flow cytometry. Issue 5 (1st May 2002)

• Record Type: Journal Article
• Authors: Morgan, J P; Robins, R A; Dua, H S; Tighe, P J
• Is Part Of: British journal of ophthalmology. Volume 86:Issue 5(2002)
• Page Start: 517
• Page End: 520
• Publication Date: 2002-05-01
• Subjects: uveoretinitis -- cytokine -- flow cytometry -- S antigen; CFC, cytokine flow cytometry -- EAU, experimental autoimmune uveoretinitis -- PBMC, peripheral blood mononuclear cell -- PCR, polymerase chain reaction -- PMA, phorbol myristate acetate -- SAg, retinal S antigen; Ophthalmology -- Periodicals; 617.7
• DOI: 10.1136/bjo.86.5.517
• Languages: English
• ISSNs: 0007-1161

Abstract

Abstract : Background/aims: Effector T cell activation is particularly important in the initiation of autoimmune uveitis. This pilot study seeks to demonstrate activation of human peripheral effector T cells in response to the uveitis candidate autoantigen, retinal S antigen (SAg), using cytokine flow cytometry (CFC). Methods: Peripheral blood mononuclear cell (PBMC) suspensions from uveitis patients and controls were stimulated with bovine SAg. Activation responses were detected by CFC. Results: Electronic gating enabled analysis of CD69+, IFN-γ+ CD4+ lymphocytes. An SAg specific response was detectable in four of 13 patients and four of eight controls. Conclusion: SAg specific, peripheral, effector T cell activation can be detected by CFC. Similar levels of responsiveness were seen in patient and control groups. More detailed cytokine profiling may demonstrate functional differences between the groups.