Histopathological analysis in experimental macular surgery with trypan blue. Issue 9 (17th August 2004)

• Record Type: Journal Article
• Authors: Grisanti, S; Szurman, P; Tatar, O; Gelisken, F; Aisenbrey, S; Oficjalska-Mlynczak, J; Kaczmarek, R; Bartz-Schmidt, K U
• Is Part Of: British journal of ophthalmology. Volume 88:Issue 9(2004)
• Page Start: 1206
• Page End: 1208
• Publication Date: 2004-08-17
• Subjects: BSS, balanced salt solution -- ERM, epiretinal membrane -- ILM, internal limiting membrane; trypan blue -- vital dye staining -- macular surgery -- internal limiting membrane -- epiretinal membranes; Ophthalmology -- Periodicals; 617.7
• DOI: 10.1136/bjo.2004.042101
• Languages: English
• ISSNs: 0007-1161

Abstract

Abstract : Aim: To analyse the effect of trypan blue on the retina in an experimental setting of macular surgery. Methods: Porcine eyes were used within 3 hours after death. The eyes were hemisected and the vitreous removed. Trypan blue (0.15%) was applied over the trephined posterior pole, whereas the rest of the eye cup was filled with a balanced salt solution (BSS). The dye and the BSS were removed after 1 minute and the complete eye cup irrigated and filled with fresh BSS. Both the treated and untreated retinas were illuminated with a standard surgical light pipe and source at maximum power for 10 minutes. Both the trypan blue exposed retina and the non-treated surrounding retina were processed for histology. Results: Exposure of the retina to trypan blue for 1 minute, followed by illumination caused no histologically detectable damage compared to the controls. No microarchitectural disorganisation, cellular disruption, or affection of the vitreoretinal interface was detected. Conclusions: These findings indicate that a 1 minute exposure of trypan blue followed by illumination does not cause an acute morphologically detectable toxic effect on the porcine retina.