Investigation of adipogenic effects of human adenovirus serotypes 36 and 5 in a Colo-320 cell line. (June 2013)

• Record Type: Journal Article
• Authors: Sanlidag, Tamer; Akcali, Sinem; Vatansever, Seda; Cicek, Candan; Sayan, Murat; Cakmakliogullari, Elcin Kal; Taskiran Sen, Pinar
• Is Part Of: Future virology. Volume 8:Number 6(2013)
• Page Start: 617
• Page End: 622
• Publication Date: 2013-06
• Subjects: adenovirus serotypes -- adipogenic effect -- cell line -- electron microscopy -- immunohistochemical method; Virology -- Periodicals; Virus diseases -- Treatment -- Periodicals; 616.9101
• DOI: 10.2217/fvl.13.39
• Languages: English
• ISSNs: 1746-0794

Abstract

Aim: We aimed to investigate the adipogenic effects of adenovirus (Ad) serotypes 5, 36 and 8 in a Colo-320 cell line using histochemical, immunohistochemical and electron microscopic methods.Materials & methods: Ad serotypes were inoculated in a Colo-320 cell line and were cultured for 14 days. They were then collected and fixed with 4% paraformaldehyde to analyze their adipogenic effects using histochemistry, immunohistochemistry and electron microscopic methods. Intracellular lipid droplets were detected in the Colo-320 cells inoculated with Ad36 and Ad5 by electron microscopic analyses.Results: After Oil Red O staining, the pink–orange staining was positive intracellularly in Colo-320 cells infected with Ad36 and Ad5. In addition, the leptin immunoreactivity was also positive in these cells.Conclusion: Our results suggested that intracellular lipid accumulation occured after infection with Ad36 and Ad5. The positive staining of Oil Red O and leptin also supported the electron microscopic results; therefore, we conclude that this accumulation occurred due to adipogenic effects of Ad36 and Ad5.