Quantification of co-trimoxazole in serum and plasma using MS/MS. (November 2015)

• Record Type: Journal Article
• Authors: Dijkstra, Jacob A; Alsaad, Noor SI; Hateren, Kai van; Greijdanus, Ben; Touw, Daan J; Alffenaar, Jan-Willem C
• Is Part Of: Bioanalysis. Volume 7:Number 21(2015)
• Page Start: 2741
• Page End: 2749
• Publication Date: 2015-11
• Subjects: Drugs -- Analysis -- Periodicals; 615.19005
• DOI: 10.4155/bio.15.188
• Languages: English
• ISSNs: 1757-6180

Abstract

Background: Co-trimoxazole is frequently used in the prophylaxis and treatment of Pneumocystis carinii pneumonia. High plasma concentrations of sulfamethoxazole or trimethoprim are correlated with toxicity. There is, however, a large variation in PK observed which can lead to underexposure or toxicity.Results: We developed a novel LC–MS/MS method to analyze the components of co-trimoxazole, trimethoprim and sulfamethoxazole and its metabolite sulfamethoxazole- N -acetyl. This new method is expeditious due to its limited sample preprocessing and a relatively short run-time of only 3 min.Conclusion: This new method met the US FDA requirements on linearity, selectivity, precision, accuracy, matrix effects, recovery and stability and is suitable for routine analysis and future prospective studies.