An UPLC–MS detection method for the quantification of five antibiotics in human plasma. (September 2015)

• Record Type: Journal Article
• Authors: Chahbouni, Abdel; den Dungen, Frank AM van; Vos, René M; den Burger, Jeroen CG; Sinjewel, Amo; Wilhelm, Abraham J; Veldkamp, Agnes I; Swart, Eleanor L; van Weissenbruch, Mirjam M
• Is Part Of: Bioanalysis. Volume 7:Number 18(2015)
• Page Start: 2321
• Page End: 2329
• Publication Date: 2015-09
• Subjects: Drugs -- Analysis -- Periodicals; 615.19005
• DOI: 10.4155/bio.15.121
• Languages: English
• ISSNs: 1757-6180

Abstract

Background: An UPLC–MS detection method for the quantification of amikacin, flucloxacillin, meropenem, penicillin G and vancomycin was developed and validated.Results: The calibration curves were found to be linear from 0.47 to 50 mg/l for amikacin, 1.28 to 135 mg/l for flucloxacillin, 0.75 to 80 mg/l for meropenem, 0.38 to 80 mg/l for penicillin G and 0.73 to 80 mg/l for vancomycin. Between- and within-run accuracy was ranged between 85 and 115%. Between and within imprecision expressed as CV was within 15%.Conclusion: The validated method was successfully applied to a PK study in very preterm and small for gestational age infants treated for nosocomial sepsis and/or meningitis.