An UHPLC–MS/MS method for the simultaneous determination of ampicillin and sulbactam in human plasma and urine. (September 2015)

• Record Type: Journal Article
• Authors: Parker, Suzanne L; Adnan, Syamhanin; Ordóñez Meija, Jenny L; Paterson, David L; Lipman, Jeffrey; Roberts, Jason A; Wallis, Steven C
• Is Part Of: Bioanalysis. Volume 7:Number 18(2015)
• Page Start: 2311
• Page End: 2319
• Publication Date: 2015-09
• Subjects: Drugs -- Analysis -- Periodicals; 615.19005
• DOI: 10.4155/bio.15.143
• Languages: English
• ISSNs: 1757-6180

Abstract

Background: With few new antibiotics available and an increased prevalence of multidrug-resistant bacteria, optimizing the effectiveness of currently available antibiotics and minimizing the potential for emerging resistance, through PK characterization, is of paramount importance.Results: Inter- and intra-assay results for ampicillin were within 5% for accuracy and 7% for precision, and for sulbactam within 7% for accuracy and 7% for precision, for both plasma and urine. The plasma Cmax was 38.4 µg/ml for ampicillin and 19.0 µg/ml for sulbactam. The urine concentrations were 668 µg/ml for ampicillin and 438 µg/ml for sulbactam.Conclusion: An UHPLC–MS/MS method to simultaneously measure co-formulated ampicillin and sulbactam in plasma and urine, for use in a patient PK study, has been developed and validated.