A new DBS card with spot sizes independent of the hematocrit value of blood. (September 2015)

• Record Type: Journal Article
• Authors: Mengerink, Ynze; Mommers, John; Qiu, Jun; Mengerink, Josh; Steijger, Odile; Honing, Maarten
• Is Part Of: Bioanalysis. Volume 7:Number 16(2015)
• Page Start: 2095
• Page End: 2104
• Publication Date: 2015-09
• Subjects: Drugs -- Analysis -- Periodicals; 615.19005
• DOI: 10.4155/bio.15.133
• Languages: English
• ISSNs: 1757-6180

Abstract

Background: DBS cards have been a big promise for decades. However, blood with low hematocrit (Ht) values results on regular cellulose-based DBS cards in larger spot sizes, compared with blood with high Ht-values. A new material has been developed to solve this problem.Results: This material, based on hydrophilic-coated woven polyester fibers, shows spot sizes independent of the Ht-value of blood. Homogeneity over the spot is within 10% RSD.Conclusion: Quantitative measurements over a broad Ht range show nonbiased results compared with whole spot analysis. The cards are experienced as reproducible, robust and easy to use on aspects of punchability and extractability.