A disposable sampling device to collect volume-measured DBS directly from a fingerprick onto DBS paper. (September 2015)

• Record Type: Journal Article
• Authors: Lenk, Gabriel; Sandkvist, Sören; Pohanka, Anton; Stemme, Göran; Beck, Olof; Roxhed, Niclas
• Is Part Of: Bioanalysis. Volume 7:Number 16(2015)
• Page Start: 2085
• Page End: 2094
• Publication Date: 2015-09
• Subjects: Drugs -- Analysis -- Periodicals; 615.19005
• DOI: 10.4155/bio.15.134
• Languages: English
• ISSNs: 1757-6180

Abstract

Background: DBS samples collected from a fingerprick typically vary in volume and homogeneity and hence make an accurate quantitative analysis of DBS samples difficult.Results: We report a prototype which first defines a precise liquid volume and subsequently stores it to a conventional DBS matrix. Liquid volumes of 2.2 µl ± 7.1% (n = 21) for deionized water and 6.1 µl ± 8.8% (n = 15) for whole blood have been successfully metered and stored in DBS paper.Conclusion: The new method of collecting a defined volume of blood by DBS sampling has the potential to reduce assay bias for the quantitative evaluation of DBS samples while maintaining the simplicity of conventional DBS sampling.